Part:BBa_I742105
Sam5 gene with ribosome binding site
This part includes the sam5 gene of the bacterium Saccharothrix espanaensis DSM 4429 along with its native ribosome binding site. The coding sequence alone, with no ribosome binding site, as also been submitted as BBa_I742142. Sam5 encodes a 4-coumarate 3-hydroxylase. The substrate is p-coumaric acid, which is converted to caffeic acid. This gene is naturally involved in caffeic acid biosynthesis (Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)) and is part of the artifical vanillin biosynthesis pathway devised by the Edinburgh iGEM2007 team.
Please note that this version of the gene contains a PstI site near the 3' end of the coding sequence. This will be removed by site directed mutagenesis in due course, at which time this entry will be edited to reflect the availability of the revised part. The present version, including the PstI site, was prepared so as to test activity prior to undertaking the mutagenesis. It can only be inserted downstream of another biobrick (as a EcoRI-SpeI insert) or have another biobrick inserted downstream of it.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1436
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1436
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1436
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1436
Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 45
Illegal NgoMIV site found at 795
Illegal NgoMIV site found at 968
Illegal NgoMIV site found at 1370
Illegal NgoMIV site found at 1374 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 569
| None |